Analysis Note

ControlHuman Cell lysate - whole cell (MDA-MB231 Cell Line)

Application

Western blot:
1:500 - 1:2,000 dilution from a previous lot was used. HeLa or Jurkat whole cell lysate can be used as a positive control and 80 to 130 kDa bands can be detected which may represent mature protein, precursor, and glycosylated TACE.

Immunocytochemistry:

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Detect ADAM 17 using this Anti-ADAM 17 Antibody validated for use in Western blotting and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ADAM17, also known as TNF-alpha Convertase, or TACE, was first cloned from human epithelial cells. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26 kDa Type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). The ADAM proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane, and a cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metallo-proteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.

Immunogen

Raised against a peptide corresponding to amino acids 807-823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid.(3).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Format: Purified

Quality

Routinely evaluated by Immunocytochemistry on A431 cells.

Immunocytochemistry Analysis: Confolcal fluorescent analysis of A431 using rabbit anti-ADAM 17 polyclonal antibody.

Specificity

TACE is a membrane-bound metallo-proteinase-disintegrin. ADAM 17 also processes other cell surface proteins, including TNF receptor, TGF alpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). ADAM 17 mRNA is expressed in a variety of human and murine tissues.

Storage and Stability

Stable 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

80 to 130 kDa